Method for the preparation of cholesterol oxidase

Details Method for the preparation of cholesterol oxidase Method for the preparation of cholesterol oxidase

C - Chemistry, Metallurgy

12

P

195/123

C12P 21/00 (2006.01) C12N 9/04 (2006.01)

Patent

CA 1069073

Abstract of the Disclosure A method for preparing cholesterol oxidase, com- prising the steps of: a) growing a cholesterol oxidase producing microorganism in a medium comprising a nontoxic concentration of nonionic surfactant to yield extracellular cholesterol oxidase; and b) separating the extracellular cholesterol oxidase from the medium. Preferably, cholesterol oxidase is prepared from a growth medium of Nocardia cholesterolicum species NRRL 5767 or 5768 which contains cholesterol, a suitable derivative of cholesterol or a 3-.beta.-hydroxy sterol as a cholesterol oxidase inducer and a nonionic surfactant in a concentration which is not toxic to said bacterium, which does not produce decomposi- tion products in concentrations sufficient to be toxic to said bacterium, and in sufficient concentration to produce extra- cellular enzyme. - 1 -

262931

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