G - Physics – 01 – N
Patent
G - Physics
01
N
73/103, 35/14, 7
G01N 33/48 (2006.01) G01N 1/10 (2006.01) G01N 23/00 (2006.01) G01N 33/49 (2006.01) G01T 7/02 (2006.01)
Patent
CA 1055724
ABSTRACT OF THE DISCLOSURE Laboratory investigations in vitro of destruction and damage of living cells frequently involve labeling of the tissue cells in question with a radioactive isotope and subsequent dispersion of the cells in a suit- able liquid. The dispersion is allowed to settle in a test tube, whereby a column of supernatant liquid is formed above precipitated cell particles. The radioactivity of said supernatant is considered a measure of cell destruct- ion and in order to measure the same completely separated from the precipi- tated cell tissue, the total amount or an accurately known fraction of the liquid must be collected, at the same time avoiding any collection of pre- cipitated particles. In order to secure this it is suggested according to the present invention that an actively absorbing surface of a liquid absorb- ent body is immersed into said amount of liquid in the container with a filter permeable to said liquid, but not to said particles, completely covering said surface, and said amount of liquid is allowed to be completely absorbed by the liquid absorbent body through said filter, which subsequently is separat- ed from said actively absorbing surface before the absorbent body is removed from the container. Utilising an absorbent body with sufficient absorbing capacity to assure a complete inclusion of the supernatant liquid in said body, with the exclusion of all cell particles by means of said filter, the total leakage of radioactivity from the cells to the liquid may be measured with high reliability by means of a subsequent scintillation counting.
249426
Hirschberg Henry
Skare Helge
Hirschberg Henry
Skare Helge
LandOfFree
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