Beta-galactosyl-umbelliferone-labeled protein and...

C - Chemistry – Metallurgy – 07 – H

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530/15.06

C07H 17/075 (2006.01) C07H 21/00 (2006.01) G01N 33/533 (2006.01)

Patent

CA 1152490

ABSTRACT OF THE DISCLOSURE A .beta.-galactosyl-umbelliferone-labeled conjugate of the formula: Image wherein ?NH)L is a protein or polypeptide, such as an immuno- globulin, bound through an amino group thereof, n is an integer from 2 through 10, m is an integer from 1 through 10, and ? is on the average from 1 to the number of available amino groups in L. An intermediate in the synthesis of such labeled conjugate is also provided. The libeled conjugates are useful as reagents in specific binding assays (e.g., immunoassays) for determining the conjugated protein or poly- peptide, or a specific binding analog or partner thereof, in liquid media.

359111

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