C - Chemistry – Metallurgy – 07 – D
Patent
C - Chemistry, Metallurgy
07
D
260/315.05
C07D 233/72 (2006.01)
Patent
CA 1137988
ABSTRACT OF THE DISCLOSURE Reagents for use in binding assays, particularly immuno- assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including .beta.-galactosyl-umbelliferone- diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N1, N3 and .sigma.-phenyl derivatives of diphenylhydantoin for preparing same. N1 and N3-.omega.- aminoalkyl and .sigma.-(.omega.-aminoalkoxy)- phenyl derivatives are prepared and coupled by amide linkage to a .beta.-galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N1 and N3-.omega.-carboxyalkyl and .sigma.-(.omega.-carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic poly- amino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.
393746
Miles Laboratories Inc.
Osler Hoskin & Harcourt Llp
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