C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.17, 195/1.
C12N 15/00 (2006.01) C07K 14/195 (2006.01) C12N 1/20 (2006.01) C12N 15/74 (2006.01)
Patent
CA 1192509
ABSTRACT Bacteria of the genus Rhizobium nodulate legumes and are responsible for nitrogen fixation. The energetics of this process are improved if hydrogen uptake ability (Hup) is imparted to the bacteria to "recycle" some of the hydrogen lost during the nitrogen fixation. Such Hup is observed in a naturally occurring strain of Rhizobium leguminosarum but it was not known how to transfer it to other strains. The present invention provides recombinant plasmids pIJ1008 and pIJ1007 which enable it to be transferred. These plasmids are formed from a transmissible plasmid, synthesised to include a drug-resistance marker, and a plasmid of the naturally occurring strain. Cultures containing the plasmids have been deposited in a culture collection and can he used, after appropriate further crosses as described, to impart Hup to other strains of Rhizobium. In addition, cultures containing the starting trans- missible plasmids (pVW5JI and pVW3JI) have been deposited and can be used to prepare strains of Rhizobium containing the same or similar recombinant plasmids. Plant growth analysis in laboratory tests demonstrate that rhizobial strains containing these recombinant nodulation plasmids which confer Hup+ ability are superior to the corresponding Rhizobium field isolates in terms of overall plant growth dependent on symbiotic nitrogen fixation.
413627
Brewin Nicholas J.
Johnston Andrew W.b.
Fetherstonhaugh & Co.
National Research Development Corporation
LandOfFree
Synthetic plasmid and bacteria containing it does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Synthetic plasmid and bacteria containing it, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthetic plasmid and bacteria containing it will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1167989