Immunoassay or nucleic acid assay with gas phase detection...

Details Immunoassay or nucleic acid assay with gas phase detection... Immunoassay or nucleic acid assay with gas phase detection...

G - Physics

01

N

167/37, 150/3.3,

G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1259911

ABSTRACT IMMUNOASSAY OR NUCLEIC ACID ASSAY WITH GAS PHASE DETECTION OF VOLATILE TAG At the conclusion of a selective binding assay (e.g., immunological or nucleic acid), an enzyme is present in modulated concentration and/or activity to moderate a chemical reaction such as the cleavage of o- nitrophenyl-b-D-galactopyranoside. After a controlled period, the enzymatic product is transferred to the gas phase and concentrated relative to other components in the enzymatic reaction mixture, such as by extraction into ethyl ether, injection into a gas chromatography column and detection by flame ionization or electron capture. Kits for such assays are also disclosed.

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