Simplified method for determining the degree of freshness of...

G - Physics – 01 – N

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324/23, 150/15.1

G01N 33/12 (2006.01)

Patent

CA 1230807

- 1 - Title of the Invention Simplified Method for Determining the Degree of Freshness of Fish and Mollusks Abstract of the Disclosure Disclosed is a method for determining the degree of freshness of raw, frozen and processed fish and mollusks. The degree of freshness can be easily determined by the method of the present invention in shorter periods of time than by the conventional methods. The method of this invention comprises practi- cably determining the index of freshness of fish and mollusks, including shellfish, which index is well known in the art and is expressed as the ratio of the combined molar concentration of inosine and hypoxanthine to the total molar concentration of the decomposition products of adenosine triphosphate wherein each concentration is determined electrochemically based on the consumption of dissolved oxygen or the amount of hydrogen peroxide produced when a sample is subjected to action by certain enzymes, i.e., inosine and hypoxanthine by nucleoside phosphorylase and xanthine oxidase, and the decomposi- tion products of adenosine triphosphate by crude alkaline phosphatase, nucleoside phosphorylase and xanthine oxidase.

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