Bioassay for chemicals which generate prooxidant states

Details Bioassay for chemicals which generate prooxidant states Bioassay for chemicals which generate prooxidant states

C - Chemistry, Metallurgy

12

Q

150/15, 73/55

C12Q 1/26 (2006.01) C12Q 1/00 (2006.01) C12Q 1/32 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1339059

A bioassay making use of submitochondrial particles to test for the presence of toxic substances which induce prooxidant states in vivo. The assay uses complex I of the electron transport enzymes on the submitochondrial particles which are capable of donating electrons to the toxicant in solution. The presence of any activated oxygen species in the assay solution is detected spectrophotometrically by the adrenochrome reaction.

611812

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