C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3, 150/8.5
C12Q 1/68 (2006.01) C12Q 1/04 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 1192120
ABSTRACT OF THE DISCLOSURE: A microbial diagnostic method based on the hybridization of nucleic acids on a solid carrier, and a reagent combination for carrying out the method. The method which can be used to identify all the microbes or microbial groups from a single sample containing nucleic acids of the microbes or microbial groups to be diagnosed, after having first rendered said nucleic acids single- stranded, basically consists in adding to the sample two nucleic acid reagents for each microbe or microbial group to be diagnosed. One of these nucleic acid reagents consists of a nucleic acid fragment from the same microbe or microbial group, which is attached to a solid carrier in a single stranded form. The other reagent consists of a completely different nucleic acid fragment from the same microbe or microbial group, which is labelled with a given marker. The nucleic acid fragment attached to the carrier is selected to hybridize to a complementary single-stranded nucleic acid from the sample to form a hybrid on the carrier. This hybrid becomes labelled when the labelled nucleic acid fragment anneals to it. Of course, the nucleic acid reagents are selected not to hybridize to each other. The labelled hybrid attached to the carrier can be measured using established methods.
413539
Ranki Tuula M.
Soderlund Hans E.
Robic Robic & Associes/associates
Sangtec Medical Ab
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