Non-radioactively labeled microspheres and use of same to...

Details Non-radioactively labeled microspheres and use of same to... Non-radioactively labeled microspheres and use of same to...

A - Human Necessities

61

B

167/114, 8/2, 16

A61B 5/02 (2006.01) A61B 5/0275 (2006.01) A61B 5/07 (2006.01) A61K 49/00 (2006.01)

Patent

CA 1255219

ABSTRACT OF THE DISCLOSURE A safe and inexpensive method of measuring blood flow in experimental animals using non-radioactively labeled microspheres is provided. The microspheres may be comprised of a variety of materials, including latex and agarose, and may be labeled with colored dyes or by linkage to enzymes, plant enzymes being preferred because they do not occur naturally in an animal's system. After injection and circulation of the microspheres throughout the animal's system, blood flow to particular tissue may be measured by counting the number of microspheres in the tissue sample, the initial number of microspheres in the animal's blood stream having been measured shortly after injection. In the case of microspheres labeled with colored dyes, the spheres may be counted in tissue either after separation from the tissue or while still trapped in the tissue's capillaries. Techniques for separating the microspheres from blood and tissue are also provided.

502920

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