Method of determining cystic fibrosis ciliostatic factor

Details Method of determining cystic fibrosis ciliostatic factor Method of determining cystic fibrosis ciliostatic factor

C - Chemistry, Metallurgy

12

Q

150/15, 150/3.3

C12Q 1/00 (2006.01) C12Q 1/34 (2006.01) C12Q 1/40 (2006.01)

Patent

CA 1246973

ABSTRACT A method for detecting the presence of cystic fibrosis ciliostatic factor in mammalian body fluid comprises contacting an enzyme whose activity is inhibited by the factor with a substrate for the enzyme in the presence of the body fluid, the substrate being substantially resistant to reactions catalyzed by enzymes in the body fluid, whereby the substrate is converted by the enzyme at a measurable rate, and comparing the rate of substrate conversion with the rate of substrate conversion by the enzyme in the absence of cystic fibrosis ciliostatic factor.

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