Homogeneous human interleukin 2 and process thereof

Details Homogeneous human interleukin 2 and process thereof Homogeneous human interleukin 2 and process thereof

C - Chemistry, Metallurgy

07

K

167/104

C07K 14/55 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1239871

RAN 4105/69 ABSTRACT A process for preparing human interleukin 2 in homo- geneous form which process comprises in combination passing a solution of crude human interleukin 2 through a reverse phase high performance column containing methyl or octyl groups covalently bonded to silica gel whereby said human interleukin 2 is retained by said column, eluting said column under HPLC conditions with a buffered n-propanol 0-60% (v/v) gradient and pooling fractions exhibiting human interleukin 2 activity; and passing the pooled active fractions from step A above through a reverse phase high performance column containing diphenyl groups cova- lently bonded to silica gel whereby said human interleukin 2 is retained by said column, eluting said column under HPLC conditions with a buffered n-propanol 20-60% (v/v) gradient and pooling fractions exhibiting human inter- leukin 2 activity and optionally repeating step A and/or B.

436704

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