Purification procedure of tpa from crude preparations

Details Purification procedure of tpa from crude preparations Purification procedure of tpa from crude preparations

C - Chemistry, Metallurgy

12

N

195/39.1

C12N 9/64 (2006.01) C12N 9/72 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1302928

- 24 - ABSTRACT OF THE DISCLOSURE Tissue plasminogen activator (tPA) species having a specific molecular weight can be isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with hydroxyapatite and then separately eluting the adsorbed tPA species with eluents having different pHs and/or salt concentrations.

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