Sunflower regeneration through embryogenesis and organogenesis

Details Sunflower regeneration through embryogenesis and organogenesis Sunflower regeneration through embryogenesis and organogenesis

A - Human Necessities

01

G

47/4, 195/47

A01G 7/00 (2006.01) A01H 4/00 (2006.01)

Patent

CA 1259936

ABSTRACT OF THE DISCLOSURE The present invention relates to the regeneration of sunflowers via embryogenesis and organogenesis. The process comprises the steps of: (a) culturing tissue obtained from a sunflower plant on a first medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient to ensure callus formation; (b) optionally subculturing said callus on a maintenance medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient for callus maintenance; (c) subculturing said callus on a second medium which comprises mineral salts, vitamins, amino acids, sucrose and a hormone in an amount sufficient to precondition the callus; (c) subculturing said callus on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot formation, and (d) subculturing said shoot on a fourth medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure root formation, whereby plants are obtained.

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