On-column loading

C - Chemistry – Metallurgy – 12 – N

Patent

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195/139, 195/35

C12N 11/00 (2006.01) C12P 1/00 (2006.01) C12P 19/24 (2006.01)

Patent

CA 1248896

ABSTRACT OF THE INVENTION This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity of the enzyme and periodically adding fresh enzyme to maintain a constant, continuous level of activity until the maximum carrying capacity of the support is reached.

488026

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