G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/543 (2006.01) G01N 33/541 (2006.01) G01N 33/563 (2006.01) G01N 33/564 (2006.01) G01N 33/94 (2006.01)
Patent
CA 1256025
ABSTRACT A process for the immuno-chemical quanti- tative determination of an antigen-active substance with avoidance of errors due to entrainment of serum components by incubation of the substance to be determined with a binder, which consists of a labelled first antibody directed against the substance to be determined or a Fab or Fab' fragment thereof, incubation with the substance to be determined being in solid phase and separation of liquid phase; the liquid phase is contacted with a second antibody, present in solid phase, against the binder or the complex of binder and substance to be determined and cross-reacts with none of the components of the com- plex when it is directed against the complex, the solid phase is separated off and the label bound thereon is measured, a reagent for the immuno- chemical determination of the antigen-active substance is provided; by means of the method and reagent proteins and haptens can be determined with improved sensitivity and selectivity in clinical investigations.
490280
Baier Manfred
Jering Helmut
Klose Sigmar
Boehringer Mannheim G.m.b.h.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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