G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 150/15.1
G01N 33/66 (2006.01) C12Q 1/54 (2006.01)
Patent
CA 1212022
ABSTRACT Process and reagent for the determination of glucose in haemolysed blood The present invention provides a process for the determination of D-glucose in body fluids by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg++ ions and buffer and measurement of the NADPH formed, wherein whole blood is mixed with 0.01 to 0.5% weight/volume of an alkyl sulphate or sulphonate or of an alkyl-aryl sulphonate, whereby the alkyl radicals can contain 8 to 32 carbon atoms and can contain one or more hydroxyalkylamino radicals as substituents and/or can be interrupted by one or more ether oxygen atoms and the alkyl-aryl radicals can contain 8 to 18 carbon atoms in the alkyl moiety, and optionally with a preserving agent, the said further reagents then being added directly. The present invention also provides a reagent for carrying out this method, wherein it contains alkyl sulphate or sulphonate or alkyl-aryl sulphonate, whereby the alkyl radicals can contain 8 to 32 carbon atoms and can contain one or more hydroxyalkylamino radicals as substituents and/or can be interrupted by one or more ether oxygen atoms and the alkyl-aryl radicals can contain 8 to 18 carbon atoms in the alkyl moiety, and a preserving agent.
444744
Draeger Brigitte
Ziegenhorn Joachim
Boehringer Mannheim G.m.b.h.
Marcoux & Sher Swabey Mitchell Houle
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