Method of determinination of human urine kallikrein and kit...

Details Method of determinination of human urine kallikrein and kit... Method of determinination of human urine kallikrein and kit...

G - Physics

01

N

167/40

G01N 33/534 (2006.01) G01N 33/573 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1193192

ABSTRACT OF THE DISCLOSURE In a method of determination of kallikrein in urine, in which an anti-human urine kallikrein antibody, human urine kallikrein labelled with a radio- active substance and a urine specimen containing kallikrein undergo an antigen-antibody reaction, and the radioactivity of the resultant reaction complex is measured, free kallikrein in the urine specimen and free kallikrein labelled with a radioactive substance in the reaction mixture can be easily separated from the antigen antibody complex by using an anti-human urine kallikrein antibody immobilized to a vessel and carrying out the reaction in the said vessel. For this purpose a kit is provided which comprises a combina- tion of a vessel having said anti-human urine kallikrein antibody immobilized therein, human urine kallikrein labelled with a radioactive substance, standard human urine kallikrein and a buffer solution to be used for reaction.

420327

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