T4 dna fragment as a stabilizer for proteins expressed by...

Details T4 dna fragment as a stabilizer for proteins expressed by... T4 dna fragment as a stabilizer for proteins expressed by...

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 1/20 (2006.01) C07K 14/01 (2006.01) C07K 14/565 (2006.01) C12N 15/00 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1207253

T4 DNA FRAGMENT AS A STABILIZER FOR PROTEINS EXPRESSED BY CLONED DNA Abstract of the Disclosure A DNA fragment isolated from the DNA of T-even bacteri- ophage which suppresses the degradation of abnormal proteins in bacterial cells is disclosed. The DNA fragment can be isolated by treating the DNA of T-even bacteriophage with a restriction enzyme to cleave the DNA into fragments, at least one of said fragments consist- ing essentially of a sequence of nucleotide bases which code for protein that functions to suppress degradation of abnormal proteins in bacterial cells, inserting the DNA fragments into cloning vehicles, such as plasmids, to produce hybrid cloning vehicles, transforming bac- terial cells by introducing the hybrid cloning vehicles therein, and selecting the transformants which exhibit suppressed protein degradation mechanisms. The DNA fragment can then be isolated from the selected trans- formants. The stabilizing DNA fragment can be intro- duced into bacterial host cells to increase the yield of foreign proteins expressed by cloned DNA.

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