High level production of bovine growth hormone

Details High level production of bovine growth hormone High level production of bovine growth hormone

C - Chemistry, Metallurgy

12

N

195/1.32

C12N 15/00 (2006.01) C07K 14/61 (2006.01) C12N 15/73 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1279591

21 ABSTRACT OF THE DISCLOSURE A high density fermentation process for high yield production of bovine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for bovine growth hormone under the control of a bacteriophage lambda promoter-operator and a plasmid containing the .lambda.cI857 gene which codes for the temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept at 26-30°C. Production of bovine growth hormone is then induced by raising the temperature of the medium to at least about 42°C. The temperature is then reduced to about 38°C to 41°C to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.

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