Electrochemical determination of orthophosphoric monoester...

Details Electrochemical determination of orthophosphoric monoester... Electrochemical determination of orthophosphoric monoester...

G - Physics

01

N

324/23, 150/15.2

G01N 27/52 (1985.01)

Patent

CA 1192613

- 34 - ELECTROCHEMICAL DETERMINATION OF ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE ACTIVITY (EC 3.1.3.1 AND EC 3.1.3.2: ALKALINE AND ACID PHOSPHATASES) ABSTRACT OF THE DISCLOSURE A method is disclosed for the measurement of phosphate ester substrate containing a nitro group is re- acted with acid or alkaline phosphatase under appropriate reaction conditions and the hydrolytic process is monitor- ed at electrodes which measure the current produced by the reduction of the nitro groups of the product and/or sub- strate. Alternatively, an aromatic phosphate ester sub- strate containing an amino group is reacted with acid or alkaline phosphatase under appropriate reaction conditions with the hydrolytic process being monitored at solid elec- trode which measure the current produced by the oxida- tion of the amino groups of the product and/or substrate.

422078

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