Transaminase, the preparation thereof and the use thereof

Details Transaminase, the preparation thereof and the use thereof Transaminase, the preparation thereof and the use thereof

C - Chemistry, Metallurgy

12

N

195/123, 195/134

C12N 9/10 (2006.01) C12N 11/08 (2006.01) C12P 13/00 (2006.01) C12P 13/04 (2006.01)

Patent

CA 1340515

The invention relates to a novel transaminase, the preparation thereof and the use thereof. It has been found that it is possible to isolate from E. coli DH-l a transaminase with the aid of which it is possible with great efficiency to prepare L-phosphinothricin and .gamma.-aminobutyric acid from the appropriate precursors by transferring the amino group from glutamate. The novel transaminase has a molecular weight of 25,000 to 100,000 Dalton, an isoelectric point at a pH between 3.5 and 5.5 and a substrate specificity towards L-phosphinothricin, .gamma.-aminobutyric acid and glutamate as amino-group donor as well as the appropriate keto compounds as amino acceptor.

601591

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