Method of removing dodecyl sulfate from sodium dodecyl...

Details Method of removing dodecyl sulfate from sodium dodecyl... Method of removing dodecyl sulfate from sodium dodecyl...

C - Chemistry, Metallurgy

07

K

530/3.24

C07K 1/30 (2006.01)

Patent

CA 1328539

ABSTRACT Guanidine hydrochloride (GCl) is used to remove excess sodium dodecyl sulfate (SDS) from SDS-solubilized protein solutions, and particularly from SDS-solubilized inclusion body solutions. GCl is added to the solution containing SDS to induce the formation of a GCl-SDS complex (GDS) which, when allowed to precipitate, can easily be removed by centrifugation, filtration, or other suitable means.

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