Stable dna constructs containing as selectable markers,...

Details Stable dna constructs containing as selectable markers,... Stable dna constructs containing as selectable markers,...

C - Chemistry, Metallurgy

12

N

195/1.17, 195/1.

C12N 15/00 (2006.01) C07K 14/15 (2006.01) C07K 14/49 (2006.01) C07K 14/81 (2006.01) C12N 9/90 (2006.01) C12N 15/64 (2006.01) C12N 15/67 (2006.01) C12N 15/81 (2006.01)

Patent

CA 1285505

ABSTRACT Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA mole- cule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.

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