Signal enhancement in immunoassay by modulation of enzymatic...

Details Signal enhancement in immunoassay by modulation of enzymatic... Signal enhancement in immunoassay by modulation of enzymatic...

G - Physics

01

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167/44

G01N 33/542 (2006.01) C07C 45/52 (2006.01) C07C 45/63 (2006.01) C07C 45/67 (2006.01) C07C 49/173 (2006.01) C07C 49/245 (2006.01) C07F 9/09 (2006.01) C07F 9/12 (2006.01) G01N 33/535 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1287575

SIGNAL ENHANCEMENT IN IMMUNOASSAY BY MODULATION OF ENZYMATIC CATALYSIS ABSTRACT OF THE DISCLOSURE A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.

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