Multidrug resistance in mammalian cell lines and isolation...

Details Multidrug resistance in mammalian cell lines and isolation... Multidrug resistance in mammalian cell lines and isolation...

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/12 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 1290712

27 WP:JW9 ABSTRACT A method for isolating a cDNA specific for P-glycoprotein is disclosed. The P-glycoprotein is the determinant in the multidrug resistance phenotype exhibited by living cells. The nucleic acid equivalent of the cDNA may be cloned in a recombinant plasmid or phage. The cDNA may also be used as a probe in determining multidrug resistance in cells. The DNA molecules specific for the P-glycoprotein is in the form of a gene family where various members of the gene family are amplified to varying degrees in multidrug resistant cells from various sources.

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