Assay process with non-boiling denaturation

Details Assay process with non-boiling denaturation Assay process with non-boiling denaturation

G - Physics

01

N

150/11, 167/40

G01N 33/68 (2006.01) G01N 33/60 (2006.01) G01N 33/82 (2006.01)

Patent

CA 1179259

ABSTRACT OF THE DISCLOSURE Competitive protein binding radioassay for sera (or cell) vitamin 812 and/or serum folate (target components, or analytes) or other target components in liquid ambient utilizing a highly alkaline (pH 12-14) environment and reducing agent for denaturing separating the target component(s) from serum without boiling, consistent with other requirements of such assays, then reducing pH to an a-10 range for effecting the competitive protein binding after which protein bound and unbound groups of radioactively tagged replicates of the target component(s) can be separated and detected to determine content of the target component(s) in the original serum (or cell), i.e. original endogenous analyte(s).

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