Method for producing a heterologous protein in insect cells

Details Method for producing a heterologous protein in insect cells Method for producing a heterologous protein in insect cells

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 9/72 (2006.01) C12N 15/11 (2006.01) C12N 15/866 (2006.01)

Patent

CA 1330426

METHOD FOR PRODUCING A HETEROLOGOUS PROTEIN IN INSECT CELLS Abstract of the Invention A mixed composition polyhedral inclusion body (PIB) is provided which contains a mixture of nucleocapsids of at least two genetically distinct baculoviruses. At least one of the baculoviruses is genetically engineered to contain at least one heterologous gene. Followed ingestion of the mixed composition PIB by an insect host, a mixed viral infection ensues in the insect permitting the production therein of additional copies of the mixed composition PIB and the production of a heterologous protein encoded by the heterologous gene present in at least one of the baculoviruses.

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