Methods of using chemiluminescent 1,2-dioxetanes

Details Methods of using chemiluminescent 1,2-dioxetanes Methods of using chemiluminescent 1,2-dioxetanes

G - Physics

01

N

150/15, 167/43,

G01N 33/53 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/531 (2006.01) G01N 33/535 (2006.01) G01N 33/573 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1340070

Processes are disclosed in which light of different wavelengths is simultaneously released from two or more enzymatically decomposable chemiluminescent 1,2-dioxetane compounds, said compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of them, to each emit light of said different wavelengths, by decomposing each of said compounds by means of a different enzyme. Such processes can be used in multi-channel assays -- immunoassays, chemical assays and nucleic acid probe assays -- to detect the presence or determine the concentration of chemical or biological substances, and in multi-channel chemical/physical probe procedures for studying the microstructures of macromolecules.

604311

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