Dry culture media

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/16 (2006.01) C12M 1/26 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 1183094

Abstract A device for culturing microorganisms is described comprising a body member comprising a self- supporting, water-proof substrate; a layer of adhesive coated on the substrate, the adhesive being non-inhibitory to the growth of microorganisms; and a cold-water soluble powder adhered uniformly to the surface of the adhesive, the powder comprising a gelling agent and/or nutrients for growing microorganisms. Another device is described comprising a body member comprising a self-supporting, water-proof substrate; a coating coated directly on the substrate, the coating being substantially water-free and consisting essentially of a cold-water-reconstitutable material comprising a gelling agent and/or nutrients for growing microorganisms; and a cover sheet releasably adhered to the bottom member, the cover sheet being substantially impermeable to bacteria and water vapor. Use of conventional agar medium is particularly inconvenient and time-consuming since the agar must be boiled and then carefully cooled to 45°C prior to pouring into petri dishes. The devices of the present invention, on the other hand, comprise media which is activated by the addition of water or an aqueous test sample at room temperature.

394891

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