Immobilized enzyme preparation and its use

Details Immobilized enzyme preparation and its use Immobilized enzyme preparation and its use

C - Chemistry, Metallurgy

12

N

195/134, 195/35.

C12N 11/02 (2006.01) C12N 9/96 (2006.01) C12N 11/08 (2006.01) C12N 11/14 (2006.01) C12P 13/04 (2006.01) C12P 41/00 (2006.01)

Patent

CA 1298800

ABSTRACT A stabilised enzyme preparation comprising an immobilised hydantoinase is useful for the preparation of a D(-)(optionally substituted phenyl)glycine or N-carbamoyl derivative thereof by hydrolysis of a 5-(optionally substituted phenyl)hydantoin. The enzyme preparation comprises cells or treated cells of a hydantoinase - producing organism immobilised within or on a suitable solid support, or an enzyme derived from the organism adsorbed on or bonded to a polymeric support, together with divalent metal ions which stabilise the preparation and render it capable of re-use. Processes for preparing the enzyme preparation and for using it to prepare commercially important end products such as D(-)(4-hydroxyphenyl)glycine are described.

546904

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