Polynucleotede dertermination with selectable cleavage sites

Details Polynucleotede dertermination with selectable cleavage sites Polynucleotede dertermination with selectable cleavage sites

C - Chemistry, Metallurgy

12

Q

260/462, 195/1.1

C12Q 1/68 (2006.01) C07F 9/02 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01)

Patent

CA 1340231

Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Particularly, various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support, where the release of the label can be detected as indicative of the presence of a particular oligonucleotide sequence in a sample. The method finds use in diagnosis of disease, genetic monitoring, and analysis of nucleic acid mixtures.

579309

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