Method for making genetically modified micro- organisms

Details Method for making genetically modified micro- organisms Method for making genetically modified micro- organisms

C - Chemistry, Metallurgy

12

N

99/50, 195/1.14,

C12N 1/20 (2006.01) C12N 1/32 (2006.01) C12N 9/06 (2006.01) C12N 15/00 (2006.01) C12N 15/74 (2006.01) C12N 15/78 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1187011

Abstract A method for making a genetically modified microorganism wherein DNA material, particularly material comprising a gdh gene, is transferred from a donor microorganism to a recipient micro- organism using a plasmid vector. The invention relates in partic- ular to the use of multicopy plasmids and/or DNA fragments having at their ends target sites for SalI restriction enzymes. The method may involve a plurality of steps, transfer of DNA fragments into intermediate microorganisms and use of a plurality of plasmids. Genetically modified microorganisms and a process for the produc- tion of single cell protein using the genetically modified micro- organisms are also claimed.

372550

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