Hybridization assay employing labeled probe and anti- hybrid

Details Hybridization assay employing labeled probe and anti- hybrid Hybridization assay employing labeled probe and anti- hybrid

C - Chemistry, Metallurgy

12

Q

167/37

C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1250230

ABSTRACT OF THE DISCLOSURE A nucleic acid hybridization assay involving a labeled probe and formation of a hybrid having epitopes for an antibody reagent. The label provides a detectable response which is measurably different when the labeled probe is comprised in a hybrid that is bound by the antibody reagent compared to when not comprised in such a hybrid. Particularly useful antibody reagents are antibodies such as anti-DNA'RNA, anti-RNA'RNA and antibodies to intercalated duplexes which do not bind substantially to single stranded nucleic acids. Modulation of the label response can be accomplished in a variety of ways such as by steric inactivation or hindrance or by labeling the antibody reagent with a second label which interacts with the first label. The assay is particularly advantageous because no separation of hybridized and unhybridized probe is required.

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