C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
195/1.12, 150/8.
C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12N 15/11 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 1339981
The present invention provides a replicable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heterologus RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo or polynucleotide of interest. This invention also provides methods for determining the presence or concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.
596864
Kramer Fred R.
Lizardi Paul M.
Gowling Lafleur Henderson Llp
The Trustees Of Columbia University
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