Method for the detection of proteins and viruses

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1290247

ABSTRACT OF THE DISCLOSURE Proteins or viruses are detected by means of an immunoassay method in which an extended solid phase coated with antiprotein or antiviral antibody is employed to bind and remove proteins or virions from a specimen by forming an immuno-complex with antigens of said proteins or virions, a mobile solid phase comprising a dispersion of microspheres coated with the antiprotein or antiviral antibody is used to bind said microspheres to antigens associated with said immuno-complex, and the presence of bound microspheres is detected. The detection sensitivity is amplified by the ability to more readily detect the microspheres, which may be dyed or labelled. The extended solid phase advantageously may be in the form of a dipstick which can be easily contacted with the specimen. A virus detection kit provides the extended solid phase and mobile solid phases, each coated with antiprotein or antiviral antibodies.

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