Process for plant tissue culture propagation

C - Chemistry – Metallurgy – 12 – N

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47/4, 195/47

C12N 5/00 (2006.01) A01H 4/00 (2006.01)

Patent

CA 1203766

PROCESS FOR PLANT TISSUE CULTURE PROPAGATION ABSTRACT The invention provides a process for at least semi- automated plant tissue culture propagation comprising: providing plant tissue culture containing a large number of densely packed meristematic areas, automatically separating and thinning the tissue into small clusters of meristematic tissues, bulk transporting the separated tissue aseptically to a separating apparatus wherein the tissue is washed and propagules of substantially uniform size and substantially free of phytotoxic cell debris are obtained, aseptically transporting the propagules to culture vessel and distributing the propagules on the surface of a culture medium.

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