Sandwich immunoassay

Details Sandwich immunoassay Sandwich immunoassay

G - Physics

01

N

167/37

G01N 33/543 (2006.01) G01N 33/541 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1254828

ABSTRACT Immunoassay system for determining the amount of a poly- valent ligand in a liquid test sample, and based on: (a) an insoluble phase to which is bound a separation specific binding substance which does not bind said ligand; (b) a capture specific binding substance which specifi- cally binds said ligand, and which is itself specifically bound by said separation specific binding substance; and (c) a probe specific binding substance which specifi- cally bind said ligand at a site which is accessible when said ligand is bound to said capture specific binding substance, said probe specific binding substance being not substantially bound by said separation specific binding substance, and being detectable by at lease one detection procedure.

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