Production of human monoclonal antibodies by human hybridomas

Details Production of human monoclonal antibodies by human hybridomas Production of human monoclonal antibodies by human hybridomas

C - Chemistry, Metallurgy

12

N

195/1.102

C12N 5/02 (2006.01) A61K 39/395 (2006.01) C07K 16/00 (2006.01) C07K 16/08 (2006.01) C12N 5/16 (2006.01)

Patent

CA 1198066

PRODUCTION OF HUMAN MONOCLONAL ANTIBODIES BY HUMAN HYBRIDOMAS ABSTRACT The present invention provides a stable, continuous human myeloma cell line which is capable of hybridization with antibody-producing cells of humans and other animals, the cell line being a mutant of GM 1500 human B cells and being deficient in hypoxanthine phosphoribosyltransferase. The invention also comprises processes for the production of hybrid cells employing the stable, HPRT-deficient human myeloma cell line, and processes for the production of antibodies employing such hybrid cells.

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