Separation and use of density specific blood cells

Details Separation and use of density specific blood cells Separation and use of density specific blood cells

G - Physics

01

N

150/11

G01N 33/50 (2006.01) A61K 35/14 (2006.01) C12Q 1/02 (2006.01) G01N 33/49 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1298182

SEPARATION AND METHOD OF USE OF DENSITY SPECIFIC BLOOD CELLS Abstract An improved method for the detection of certain diseases and body conditions based upon the response to antigens associated with the disease or condition tested for by lymphocytes having buoyant densities of 1.0590g/cm3 to 1.0670g/cm3 and densities of 1.0690g/cm3 to 1.07309/cm3 in a continuous density gradient solution having an osmolality of 0.315 to 0.320 osm/kg at a temperature of 20°C. The response is indicated by changes in the structure of the cytoplasmic matrix of the cell as measured by intracellular fluorescein fluorescence polarization. The lymphocytes are separated from peripheral blood by centrifugation in a continuous gradient density solution having limiting density gradients which bracket the range of buoyant densities of the lymphocytes to be separated.

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