Method and kit for performing nucleic acid hybridization assays

Details Method and kit for performing nucleic acid hybridization assays Method and kit for performing nucleic acid hybridization assays

C - Chemistry, Metallurgy

12

Q

167/44, 150/8.5

C12Q 1/68 (2006.01) C12N 15/00 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/532 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1278257

ABSTRACT A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexingagent on the first probe is employed to immobilize the target- probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.

511554

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