Process for detection of selected motile organisms

C - Chemistry – Metallurgy – 12 – Q

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150/16, 167/45

C12Q 1/10 (2006.01) C12M 1/34 (2006.01) C12Q 1/02 (2006.01) G01N 33/02 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/12 (2006.01)

Patent

CA 1271707

PROCESS FOR DETECTION OF SELECTED MOTILE ORAGNISMS Abstract of the Disclosure Methods and associated devices for detecting the presence of a particular motile organism within a sample are disclosed. The sample may be derived from dry milk, raw meat, poultry or a clinical specimen. A preferred method includes inoculating a selective enrichment medium containing a chemo- tactic attractant with the sample and contacting the selective enrichment medium with a nonselective motility medium contain- ing a chemotactic attractant in a concentration less than the attractant concentration in the selective enrichment medium. Upon incubation, the motile organism metabolizes the chemo- tactic attractant, allowing the organism to move into the motility medium where it interacts with antibodies specific for the organism, thereby causing the formation of a persistent immobilization band. The methods are particularly useful in detecting Salmonella.

493781

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