Simultaneous enzyme immunoassay for detecting antigen and/or...

Details Simultaneous enzyme immunoassay for detecting antigen and/or... Simultaneous enzyme immunoassay for detecting antigen and/or...

G - Physics

01

N

167/44

G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1303494

ABSTRACT OF THE DISCLOSURE A solid-phase immunoassay is provided for determination of members of an immunological pair of such antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or "spike" of the binding pair to the test sample prior to incuba- tion of the test sample in the presence of a solid-phase ab- sorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.

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