G - Physics – 01 – N
Patent
G - Physics
01
N
167/41, 167/45
G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1340973
The invention relates to a kit for the determination of a surface antigen characteristic of a cell population or subpopulation, which comprises the following components, namely a) a solid support to which one or more monoclonal antibodies are fixed by covalent bonding or physical adsorption, the said monoclonal antibodies being directed against surface antigens of the cell to he determined, other than the said characteristic antigen; b) one or more solutions each containing a monoclonal antibody specific for the said antigen characterstic of the cell population or subpopulation to be determined, which is labeled with a radioactive probe or an enzyme probe; c) in the case of antibodies labeled with an enzyme probe, one or more solutions providing the reagents necessary for developing the activity of the enzyme; and d) if appropriate, an additional component consisting of a washing buffer solution and/or samples permitting standardization and quality control of the determination. Also disclosed is a process for immunometric assay of antigens characteristic of a cell population of subpopulation, particularly for the determination of lymphocytes T or subpopulations T4 and T8.
578730
Sanofi
Sanofi-Synthelabo
Sim & Mcburney
LandOfFree
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