Process and reagent for determining the activity of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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150/3.2, 150/15.

C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 1191772

ABSTRACT The present invention provides a process for the determination of the activity of chymotrypsin or trypsin in faeces by measurement of the rate of fission of an appropriate substrate by a faecal suspension in an aqueous or aqueous-organic medium, wherein a faecal sample is suspended in the presence of a surface-active agent. The present invention also provides a reagent for carrying out this process, comprising a surface- active agent, an enzyme substrate and an aqueous salt solution, said reagent having a pH value of from 7 to 11.

410589

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