Composition for cell culture

C - Chemistry – Metallurgy – 12 – N

Patent

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195/40, 195/46

C12N 5/02 (2006.01)

Patent

CA 1177424

ABSTRACT A cell culture medium not relying on fetal bovine serum as a necessary component is disclosed. The medium optimally comprises from about 80-90% of a chemically defined nutrient medium and from about 10-20% of a natural biological fluid which has been treated by contact with "feeder" culture of smooth muscle cells for about 24 hours. The treatment may involve only natural fluid or such fluid in admixture with nutrient medium. Thymidine is then added in an amount such that its final concentration is between 10-4M and 10-6M. Such medium has been found suitable for the growth of mammalian cells, including mammalina endothelial cells. Additionally, lots of fetal bovine serum found unsuitable to support cell growth may be treated by contact with smooth muscle cells for about 24 hours and thereby converted to acceptable lots.

386521

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