Enzymatic conversion of certain sub-type a and ab erythrocytes

Details Enzymatic conversion of certain sub-type a and ab erythrocytes Enzymatic conversion of certain sub-type a and ab erythrocytes

A - Human Necessities

61

K

195/34.7

A61K 35/18 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1222712

ABSTRACT OF THE DISCLOSURE Type 0 erythrocytes are produced from certain sub- types of A erythrocytes or type AB erythrocytes by contacting the same following equilibration of a pH of 5.6 - 5.8 with an .alpha.-N-acetylgalactosaminidase, preferably obtained from an avian liver, for periods sufficient to convert the A antigen in the erythrocyte to the H antigen. Following removal of the enzyme, the erythrocyte is re-equilibrated to a pH of 7.2 - 7.4. As a result, there is obtained 0 type erythrocytes characterized by a 60 to 90 percent ATP level based on the level of ATP in naturally occurring 0 or AB erythrocytes. Beginning with certain A cells one obtains synthetic 0 erythrocytes character- ized by a terminal .alpha.-fucose moiety, 0 antigenicity, and the absence of A antigenicity. Beginning with A2B erythrocytes, one obtains B erythrocytes by the same process characterized by the absence of A antigenicity, greater H antigenicity than naturally occurring A2B cells, the presence of B antigenicity and the aforedescribed ATP levels.

460122

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