Method for the determination of plasminogen

Details Method for the determination of plasminogen Method for the determination of plasminogen

C - Chemistry, Metallurgy

12

Q

150/15.5

C12Q 1/56 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 1316088

Abstract of the disclosure A method for the determination of plasminogen in body fluids in the presence of streptokinase using a substrate for plasmin, the action of plasmin on which results in a cleavage product which can be measured optically, which method comprises essentially simultaneous addition of streptokinase and chromogenic or fluorogenic substrate for plasmin to the body fluid, and determination of the amount of the cleavage product formed in a defined time, or of the rate of formation of the cleavage product, and from this the concentration of plasminogen, is described. When the amount of streptokinase which is used is such that the plasminogen present is in molar excess, this method can be used to determine the rate of activation of plasminogen.

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