Transient expression system for producing recombinant protein

Details Transient expression system for producing recombinant protein Transient expression system for producing recombinant protein

C - Chemistry, Metallurgy

12

N

195/1.29, 195/1.

C12N 5/10 (2006.01) C07K 14/61 (2006.01) C07K 14/64 (2006.01) C07K 14/755 (2006.01) C12N 9/72 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1340480

A method for transient production of a desired heterologous protein comprising: transfecting a eukaryotic host cell with a vector producing a trans-activating protein; transfecting the eukaryotic host cell with an expression vector comprising a stabilizing sequence downstream of a promoter and upstream of a DNA encoding the desired heterologous protein and a polyadenylation sequence downstream of which is a transcription terminatein site; culturing the transfected eukaryotic host cell under conditions favorable for production of said desired heterologous protein; and, recovering the desired protein in useful amounts within about one day to about fourteen days of transfection.

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