C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/135, 167/37,
C12N 15/31 (2006.01) A61K 39/102 (2006.01) C07K 14/285 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)
Patent
CA 1341040
A vaccine for immunizing animals, including human beings, against diseases caused by microorganisms producing an osteolytic toxin, the vaccine comprising an immunologically effective amount of a recombinant, immunogenic, detoxified Pasteurella multocida toxin or toxin analogue. The vaccine is encoded by a DNA fragment comprising a nucleotide sequence coding for a Pasteurella multocida toxin or toxin analogue, the nucleotide sequence being derived from a Pasteurella multocida genome, from a bacteriophage infectious for Pasteurella multocida, or from a plasmid or being a synthetic sequence which is inserted in an expression vector capable of replicating in a suitable host microorganism in which the said DNA fragment may be expressed in terms of production by the host microorganism of Pasteurella multocida toxin or toxin analogue when it is cultivated under suitable conditions, the produced toxin or toxin analogue optionally being subjected to posttransscriptional modifications to provide the detoxified toxin or toxin analogue. Alternatively, the nucleotide sequence coding for the toxin or toxin analogue may be modified prior to insertion into the expression vector so as to provide the expressed toxin or toxin analogue in a detoxified form. A monoclonal antibody has been raised against a Pasteurella multocida toxin which may be useful as a diagnostic agent for determining the presence of a Pasteurella multocida toxin or toxin analogue in a sample. Further-more, the said monoclonal antibody may be useful for isolating a Pasteurella multocida toxin or toxin analogue from a biological material. A further scope for a useful diagnostic agent which is provided for is the application of a labelled DNA sequence homologous with a DNA sequence coding for a Pasteurella multocida toxin or toxin analogue for the detection of Pasterualla multocida toxin-producing microorganisms. The toxin or toxin analogue per se may be usefully applied for determining the presence of antibodies against a Pasteruealla multocida toxin or toxin analogue in a sample and may also be useful as a reference standard in analytical procedures aiming at quantitative or qualitative determination of the said toxin or toxin analogue in a biological sample or intermediate or final vaccine preparations.
596452
Mccarthy Tetrault Llp
Nordisk Droge & Kemikalie A/s
Statens Veterinaere Serumlaboratorium
LandOfFree
Pasteurella vaccine does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Pasteurella vaccine, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Pasteurella vaccine will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1243056